Composite

Part:BBa_K944015

Designed by: Natasha Gómez   Group: iGEM12_Panama_INDICASAT   (2012-09-26)


Cyanide insensitive terminal oxidase cioAB and cyanide biosensor expression platform

This composite part is the combination of cyanide insensitive genes to confer to the bacteria cyanide resistance, and our cyanide biosensor model with the inducible promoter and the Red Fluorescent Protein reporter gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2441
    Illegal XhoI site found at 2329
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 870
    Illegal NgoMIV site found at 1427
    Illegal NgoMIV site found at 1779
    Illegal NgoMIV site found at 2754
    Illegal NgoMIV site found at 2995
    Illegal NgoMIV site found at 3136
    Illegal NgoMIV site found at 3378
    Illegal AgeI site found at 1735
    Illegal AgeI site found at 2287
    Illegal AgeI site found at 5430
    Illegal AgeI site found at 6210
    Illegal AgeI site found at 6322
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 560
    Illegal BsaI.rc site found at 3343
    Illegal BsaI.rc site found at 3652
    Illegal BsaI.rc site found at 5138
    Illegal SapI.rc site found at 3418


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